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pBT310_pBS-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52543

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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3186 pBS Bcl-XL Exon 1

Plasmid

#8755

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL (Exon 1) (Bcl2l1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pBS-miR106a-363

Plasmid

#19028

Depositor

Tyler Jacks

Article

Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

Insert

microRNA 106a-363 (Mir106a Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-β1L9'S-nAchR

Plasmid

#20685

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrnb1 Mouse)

Use

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Promoter

Available since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pHR-LS

Plasmid

#205438

Purpose

Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

Depositor

Masamichi Kamihira

Article

Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

Inserts

Gaussia luciferase gene
spectinomycin resistance gene

Use

Cre/Lox and Luciferase; Chlamydomonas expression

Tags

Expression

Mutation

Promoter

Heat shock protein 70A (HSP70A)/ribulose bisphosp…

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-V9

Plasmid

#173797

Purpose

pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene noctiflora ClpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation

Depositor

Daniel Sloan

Article

Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.

Inserts

aadA
ClpP1

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu, Met186ILeu ELL2

Plasmid

#127265

Purpose

For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Two Mets (138,186) to ILeu Quikchange

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met133ILeu ELL2

Plasmid

#127259

Purpose

For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133 to Ileu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Met133 to Ileu Quikchange

Promoter

T7

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge