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Plasmid#207464PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253DepositorInsertMTdTc253 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationSer253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc188
Plasmid#207463PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188DepositorInsertTdTc188 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys216Ser, Cys302Ala, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc180
Plasmid#207462PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180DepositorInsertTdTc180 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationGlu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc302
Plasmid#207461PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertTdTc302 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 bleach.2
Plasmid#191878PurposeEncodes a GFP design, bleach.2DepositorInsertbleach.2
UseTagsHis TagExpressionBacterialMutationV61L T65S V68A S72A V112I Y145MPromoterT7Available sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 mid.8
Plasmid#191902PurposeEncodes a GFP design, mid.8DepositorInsertmid.8
UseTagsHis TagExpressionBacterialMutationV61A S72T Y145M L220VPromoterT7Available sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15(CO)
Plasmid#201879PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTagsExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL9(CO)
Plasmid#201873PurposeUsed for bacterial expression of human protein ARL9DepositorInsertARL9 (ARL9 Human)
UseTagsExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPGN
Plasmid#199232PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpithelial mitogen isoform 6 (EPGN Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78
Plasmid#193367PurposeExpression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78 (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationPromoterT7Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hBTC
Plasmid#199230PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertBetacellulin (BTC Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETTEV-SENP8
Plasmid#196361Purposeprotein expression of the His-tagged full length proteinDepositorInsertSENP8/NEDP1
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78_R41A
Plasmid#193368PurposeExpression of C9ORF78 R41A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78_R41A (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationR41A, the mutation is located in the binding inte…PromoterT7Available sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsNot2_N
Plasmid#147010PurposeBacterial Expression of HsNot2DepositorInsertHsNot2 (CNOT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsPAN3_N
Plasmid#147012PurposeBacterial Expression of HsPan3DepositorInsertHsPan3 (PAN3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-DmPABPC1_J
Plasmid#146643PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutation4 silent mutations compared to the sequence geven…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:bleach.1
Plasmid#191929PurposeEncodes a GFP design, sf:bleach.1DepositorInsertsf:bleach.1
UseTagsHis TagExpressionBacterialMutationT108L Y145M T167V L220VPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only