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Spike Display_Part 1-5 DO
Plasmid#172726PurposeEncodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_Part 1 DO
Plasmid#172721PurposeEncodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_Part-5 DO
Plasmid#172725PurposeEncodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_Part 3 DO
Plasmid#172723PurposeEncodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Spike Display_Part-4 DO
Plasmid#172724PurposeEncodes a sfGFP dropout expression cassette in place of Part 4 of HexaPro-D614G spike to be used in the Spike Display systemDepositorInsertSARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…PromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA.Tetherin
Plasmid#41068DepositorInsertTetherin (BST2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
CRY2 mCherry Sec61B
Plasmid#102246PurposeExpresses fusion of CRY2PHR with mCherry and Sec61BDepositorInsertCRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pScalps_puro_mIL-2Rα RK
Plasmid#59920PurposeExpresses mouse interleukin-2 receptor alpha chain mutatedDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationchanged sequence of the intracellular (C-terminal…PromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5079_pHR_PGK_sfGFP_CoV-F1
Plasmid#155303PurposeSARS-CoV-2 fluorescent reporter 1DepositorInsertSuperfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJS699
Plasmid#168779PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombinationDepositorInsertSpike RBD (S betacoronavirus)
UseTagsCmycExpressionMutationN343QPromoterGAL1Available sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON
Plasmid#180424PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intactDepositorInsertSpike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-PLOD2_PGK-GpNLuc
Plasmid#136457PurposeExpresses Tet/Dox-inducible human PLOD2 in mammalian cells.DepositorInsertTRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR40
Plasmid#69257Purposeplin-31::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterlin-31Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR33
Plasmid#69254Purposephsp16.2::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.2Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR47
Plasmid#69258Purposepmyo-3::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promotermyo-3Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR35
Plasmid#69256Purposephsp16.41::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.41Available sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR28
Plasmid#69253Purposephlh-8::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterhlh-8Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON
Plasmid#180423PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSASDepositorInsertSpike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only
