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Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFlag, HA, and Lamp2 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-Blast-CDT1-HA
Plasmid#109335Purposevirus packaged, doxycyline inducible expression of Cdt1DepositorInsertCdt1 (CDT1 Human)
UseLentiviral; Inducible expressionTagsHA, His, StrepExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS2022
Plasmid#49129PurposeAAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.DepositorInsertssAAV-PRS2/3:Con-iCre
UseAAVTagsExpressionMammalianMutationPromoterDBHAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3F-Strep-mMALT1
Plasmid#33315DepositorInsertMalt1 (Malt1 Mouse)
UseTags3xFLAG and StrepOneExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWZ403
Plasmid#208113PurposemSwAP-In marker cassette 1 with mScarlet-PuroTKDepositorInsertmScarlet-PuroTK
UseTagsP2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad4
Plasmid#89829PurposeKnockdown human Smad4 expression in human mammalian cellsDepositorInsertSmad4 shRNA (SMAD4 Mouse, Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PKT2/CLP-AKT
Plasmid#20281DepositorInsertAKT (AKT1 Human)
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hMBOAT7-3xFLAG
Plasmid#165474PurposeHuman MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.DepositorInsertMembrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS1994
Plasmid#49124PurposeAAV plasmid with S100B (Ple266) promoter driving expression of iCre.DepositorInsertssAAV-Ple266-iCre
UseAAVTagsExpressionMammalianMutationPromoterS100BAvailable sinceMay 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-MAP1LC3A
Plasmid#137756PurposeExpression of LC3ADepositorInsertMAP1LC3A (MAP1LC3A Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
canxTM-mEmerald (cytosolic)
Plasmid#186962PurposeExpresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) and add ER signal…PromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP11C-HA
Plasmid#209220PurposeMammalian expression of ATP11CDepositorInsertATP11C (ATP11C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LiPOP2
Plasmid#168266PurposeA single-component optogenetic tool for photoswitchable pyroptosisDepositorInsertmGSDMD(1-276) (Gsdmd Mouse)
UseTagscpLOV2, mCherryExpressionMammalianMutationR138A, K146A, R152A, R154APromoterCMVAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald(luminal)-canxTM
Plasmid#186961PurposeExpresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) with ER signal pe…PromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneExpressionMutationMutated in both EF-hand domains: D231A, E242K, D4…PromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK D110A p2A mCherry
Plasmid#117694PurposeCAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs D110A peptide
UseCre/LoxTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZ854
Plasmid#208116PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-NeoDepositorInsertmNeonGreen-HPRT1-Neo
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ852
Plasmid#208114PurposemSwAP-In marker cassette 1 with mScarlet-HygTKDepositorInsertmScarlet-HygTK
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only