159,371 results

1. psgRNAc

   Plasmid

   #114006

   Purpose

   express sgRNA, p15A, CRM

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI_FLAG-GFP(Y39TAG)-6His

   Plasmid

   #223507

   Purpose

   Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

   Depositor

   Edward Lemke

   Insert

   GFP(Y39TAG)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Y39TAG

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTarget_CAST

   Plasmid

   #127926

   Purpose

   Target plasmid containing PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   PSP1 target (GGTT PAM)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF-hGPC3

   Plasmid

   #24670

   Depositor

   Jorge Filmus

   Article

   Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

   Insert

   GPC3 (GPC3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-SUMO1

   Plasmid

   #185637

   Purpose

   expression of GST-tagged SUMO1 in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

   Insert

   small ubiquitin-like modifier 1 (SUMO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-Flag-SOCS1

   Plasmid

   #129514

   Purpose

   Retroviral vector for the expression of human SOCS1 with a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAMPK alpha1 full length

   Plasmid

   #27297

   Depositor

   Morris Birnbaum

   Insert

   AMPK alpha1 (Prkaa1 Mouse)
   

   Use

   Tags

   his

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)

   Plasmid

   #221455

   Purpose

   AAV vector expressing GFAP-GeNL

   Depositor

   Maiken Nedergaard

   Article

   Beinlich et al Science. 2024 Mar 29;383(6690):1471-1478

   Insert

   Green enhanced Nano-lantern
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hGFAP

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV9-null

    Plasmid

    #200182

    Purpose

    Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV9 Cap w/ nonsense mutation at VR8
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Replaced amino acids 586-592 in Cap with nonsense…

    Promoter

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Puro-mGSDME

    Plasmid

    #154877

    Purpose

    Expresses mGSDME in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

    Insert

    GSDME (Gsdme Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Use

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161927

    Purpose

    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine repression reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIA4.721-Pansio

    Plasmid

    #214441

    Purpose

    BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    2xFKBP12-Casp9
    

    Use

    Hdr donor

    Tags

    P2A-Bsd

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXT128_TwinStrep-SUMO-ShCas12k

    Plasmid

    #135524

    Purpose

    Protein expression construct for ShCast12k

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShCas12k
    

    Use

    Tags

    TwinStrep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-NES-PhoCl-mCherry

    Plasmid

    #87690

    Purpose

    Express NES-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NES-PhoCl-mCherry
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

    Plasmid

    #86828

    Purpose

    Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1a72

    Plasmid

    #177690

    Purpose

    HCV E1E2 expression plasmid

    Depositor

    Justin Bailey

    Article

    Salas et al Gastroenterology. 2022 Feb;162(2):562-57

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge