Addgene

Showing: 1201 - 1220 of 1538 results

Results per page:

Sort results by:

D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_R1072A

Plasmid

#91859

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R1072A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

R1072A

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_K1082A

Plasmid

#91863

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1082A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1082A

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiMutB3GNT5-blast

Plasmid

#208380

Purpose

lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

Depositor

Jennifer Kohler

Insert

Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)

Use

Lentiviral

Tags

myc, FLAG

Expression

Mammalian

Mutation

nt 922 c->g (PAM site inactivation)

Promoter

EF-1 alpha core promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2144

Plasmid

#190344

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3410

Plasmid

#190410

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2462

Plasmid

#190369

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-LmrAO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-LmrAO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2461

Plasmid

#190368

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-IcaRO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2296

Plasmid

#190365

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3421

Plasmid

#190411

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFP-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFP-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2652

Plasmid

#190392

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-LacIO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-LacIO::GFPint-NLS-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2512

Plasmid

#190381

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XBetIO-min35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XBetIO-min35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3424

Plasmid

#190414

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proT::GFP-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proT::GFP-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3477

Plasmid

#190416

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XPhlFO-3XAmtRO::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

6XPhlFO-3XAmtRO::TetR-NLS-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3423

Plasmid

#190413

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proUBQ10::GFP-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proUBQ10::GFP-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB3422

Plasmid

#190412

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2500

Plasmid

#190379

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge