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pTO149_RFP_CauNEO

Plasmid

#177277

Purpose

Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

RFP, CauNEO

Use

Tags

ENO1-RFP

Expression

Bacterial

Mutation

All CUG Leucine codons were replaced with the syn…

Promoter

AgTEF2

Available since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMYO127TAG-SepT

Plasmid

#34625

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin-127TAG

Use

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized + an amber stop codon was introdu…

Promoter

Ipp

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Mutation

Promoter

Ac5

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTH848-gst-disec

Plasmid

#89589

Purpose

Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

glutathione-S transferase codon-dis-optimised for bacterial expression

Use

Tags

8xhistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCB1_STOP

Plasmid

#221424

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Insert

ABCB1 (ABCB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-SpEndoH

Plasmid

#218196

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

Depositor

Florence Pojer

Insert

EndoH

Use

Tags

tufA-His-CBD

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 45-313

Promoter

T7

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103 NEDD4 WW domain #3

Plasmid

#104210

Purpose

Bacterial expression of WW domain #3 from NEDD4

Depositor

Sachdev Sidhu, Marius Sudol

Insert

NEDD4 WW domain #3 (NEDD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 NEDD4 WW domain #1

Plasmid

#104208

Purpose

Bacterial expression of WW domain #1 from NEDD4

Depositor

Sachdev Sidhu, Marius Sudol

Insert

NEDD4 WW domain #1 (NEDD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 NEDD4 WW domain #4

Plasmid

#104211

Purpose

Bacterial expression of WW domain #4 from NEDD4

Depositor

Sachdev Sidhu, Marius Sudol

Insert

NEDD4 WW domain #4 (NEDD4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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PKMYT1

Plasmid

#39061

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PKMYT1 (PKMYT1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pBS-L1PA1CH_blast rescue

Plasmid

#69611

Purpose

Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

Depositor

Astrid Roy-Engel

Insert

L1PA1

Use

Tags

blast rescue cassette

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PBRM1

Plasmid

#39013

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PBRM1 (PBRM1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
SPR

Plasmid

#39161

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pRSET.HaloTag.v7.CoOp.Cys-less

Plasmid

#195487

Purpose

Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Insert

HaloTag.v7

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C61T, C262V

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mNeonGreen (KmR)

Plasmid

#177209

Purpose

Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

Depositor

Alexey Merz

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Yeast codon optimized

Promoter

Synthetic constitutive promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PBRM1

Plasmid

#39027

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

polybromo 1 [isoform a] (PBRM1 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103 WWP2 WW domain #1

Plasmid

#104212

Purpose

Bacterial expression of WW domain #1 from WWP2

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP2 WW domain #1 (WWP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWP2 WW domain #3

Plasmid

#104214

Purpose

Bacterial expression of WW domain #3 from WWP2

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP2 WW domain #3 (WWP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWP2 WW domain #2

Plasmid

#104213

Purpose

Bacterial expression of WW domain #2 from WWP2

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP2 WW domain #2 (WWP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 WWP2 WW domain #4

Plasmid

#104215

Purpose

Bacterial expression of WW domain #4 from WWP2

Depositor

Sachdev Sidhu, Marius Sudol

Insert

WWP2 WW domain #4 (WWP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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