Addgene

We narrowed to 2,043 results for: Xpa

Showing: 1201 - 1220 of 2043 results

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pcDNA3_SARS-CoV-Spike[BtKY72 RBD]

Plasmid

#190081

Purpose

express chimeric SARS-CoV spike with the BtKY72 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[PRD0038 RBD]

Plasmid

#190085

Purpose

express chimeric SARS-CoV spike with the PRD0038 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

DHFR P66L

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9339 (pgRNA_VII-1_NatMX)

Plasmid

#161591

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC3-TPK2

Plasmid

#176180

Purpose

yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2 (TPK2 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC6-spacer

Plasmid

#176178

Purpose

yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

41 bp non-coding DNA spacer from pYTK048

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAB-mOvol1

Plasmid

#120360

Purpose

piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

Ovol1 (Ovol1 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tet-on and CBh

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265H

Plasmid

#149554

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

hAID-ziSpyMacCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266B

Plasmid

#149555

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ziSpyMacCas9(D10A)-PmCDA1-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 351

Plasmid

#138058

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-10AG 11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG GCA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 336

Plasmid

#138057

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC 11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

GCA GAG GAG ACA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 333

Plasmid

#138056

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

GAA GAG GAG ACA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 330

Plasmid

#138055

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb- 2AC 11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

ACA GAG GAG ACA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 327

Plasmid

#138054

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC-GFP

Use

Tags

Expression

Bacterial

Mutation

GCA GAG GAG AAA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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K8.1Pr-pGL4.16-OriLyt

Plasmid

#131038

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV Lytic Origin of replication

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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