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Showing: 1201 - 1220 of 1572 results

  6. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

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  7. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3
    GNGT9
    GNA12
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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  11. pQ54-VC

    Plasmid
    #175060
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ54 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175060-plasmid-map-sequence-id-353799

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  12. pQ39-VC

    Plasmid
    #175061
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ39 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175061-plasmid-map-sequence-id-353797

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  13. pQ25-VC

    Plasmid
    #175062
    Purpose
    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    polyQ25 (HTT Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Availability
    Academic Institutions and Nonprofits only
    175062-plasmid-map-sequence-id-362344

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  14. PA-DAD-dark

    Plasmid
    #53096
    Purpose
    Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
    Depositor
    Klaus Hahn, Ronen Zaidel-Bar
    Article
    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3
    Insert
    LOV(dark)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus; C450M mutation in LOV2
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    53096-plasmid-map-sequence-id-119521

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  15. pmVen-RecQL4

    Plasmid
    #211401
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211401-plasmid-map-sequence-id-420509

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  16. pmVen-TopBP1

    Plasmid
    #211413
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211413-plasmid-map-sequence-id-420517

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  17. pmVen-RPA32

    Plasmid
    #211386
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211386-plasmid-map-sequence-id-420501

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  20. pmVen-C16orf59

    Plasmid
    #211423
    Purpose
    mammalian expression of human C16orf59 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211423-plasmid-map-sequence-id-420526

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Showing: 1201 - 1220 of 1572 results