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Plasmid#200679PurposeModular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
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pLV-EF1A-CD19.28H.28TM.BB.28.3z
Plasmid#200681PurposeModular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-BBz
Plasmid#135992PurposeModular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8h.8TM.28.3z
Plasmid#200670PurposeModular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.28.BB.3z
Plasmid#200680PurposeModular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.28.3z
Plasmid#200674PurposeModular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.BB.3z
Plasmid#200675PurposeModular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTagng-22aa
Plasmid#115345PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genomeDepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#115344PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-Traditional CAR (anti-CD19)
Plasmid#192115PurposeLentiviral delivery of Traditional CAR(anti-CD19)DepositorInsertpHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-ON VIPER CAR (anti-CD19)-final design
Plasmid#192117PurposeLentiviral deliviery of ON VIPER CAR (anti-CD19)-final designDepositorInsertpHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-R4-uAb
Plasmid#101800PurposeMammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
Plasmid#192122PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 CLV3
Plasmid#115485PurposeCRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 AP3
Plasmid#115484PurposeCRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAT_41BBICD
Plasmid#197097PurposeThis plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of 4-1BB.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAT_CD28ICD
Plasmid#197098PurposeThis plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for the intracellular domain of CD28.
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only