Addgene

Showing: 1201 - 1220 of 2422 results

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

Plasmid

#199354

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOEu

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

Plasmid

#199355

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOstar

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

Plasmid

#199365

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199366

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

Plasmid

#199368

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194897

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Availability

Academic Institutions and Nonprofits only

Enlarge
PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

Plasmid

#191691

Purpose

For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

EN1 homology arms and p2a-mTagBFP2nls-3xHAtag

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

Plasmid

#217305

Purpose

sgRNA BFP + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-BFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6, Ef1alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

Plasmid

#107855

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G368W and W385G in splicing reporter

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)

Plasmid

#107860

Purpose

To quickly insert CTD mutants into TDP43 splicing reporter

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of CTD in TDP43 splicing reporter, inser…

Promoter

Availability

Academic Institutions and Nonprofits only
pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138732

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138733

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE054 ZF43x12-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138735

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE055 ZF43x3-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138736

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE056 ZF43x6-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138737

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE057 ZF43x12-S mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138738

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge