Skip to main content
Addgene
Showing: 1201 - 1220 of 1977 results

  2. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

    Enlarge

  7. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Use
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71293-plasmid-map-sequence-id-127936

    Enlarge

  18. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    GM-CSF minimal
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

    Enlarge

  20. MICU-APEX2

    Plasmid
    #79057
    Purpose
    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Sept. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79057-plasmid-map-sequence-id-147904

    Enlarge

Showing: 1201 - 1220 of 1977 results