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Showing: 3001 - 3050 of 4441 results

  2. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

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  7. pCP-tRNA

    Plasmid
    #133812
    Purpose
    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
    Depositor
    Geraldine Butler
    Article
    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001
    Insert
    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133812-plasmid-map-sequence-id-263618

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  12. pAS088

    Plasmid
    #211502
    Purpose
    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211502-plasmid-map-sequence-id-417793

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  15. pJBL724

    Plasmid
    #140394
    Purpose
    Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-hucO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140394-plasmid-map-sequence-id-297861

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  21. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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  22. pTPH405c

    Plasmid
    #185726
    Purpose
    Directed evolution of Cas9 adenine base editors with altered PAM compatibility
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Insert
    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185726-plasmid-map-sequence-id-365709

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  24. pADH100

    Plasmid
    #90980
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90980-plasmid-map-sequence-id-180507

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  26. PB-CT3G-ERP2-MG

    Plasmid
    #175501
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    175501-plasmid-map-sequence-id-344442

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  27. PB-CT3G-CERP2

    Plasmid
    #175499
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175499-plasmid-map-sequence-id-345877

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  28. PB-CT3G-ERP2

    Plasmid
    #175497
    Purpose
    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    175497-plasmid-map-sequence-id-345451

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  30. pADH143

    Plasmid
    #90989
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90989-plasmid-map-sequence-id-180522

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  31. pADH140

    Plasmid
    #90988
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90988-plasmid-map-sequence-id-180521

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  32. pADH118

    Plasmid
    #90983
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90983-plasmid-map-sequence-id-180511

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  33. pADH137

    Plasmid
    #90986
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    90986-plasmid-map-sequence-id-180519

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  34. pADH119

    Plasmid
    #90985
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90985-plasmid-map-sequence-id-180513

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  42. PB-hEF1A-MG-U6-sgRNA

    Plasmid
    #175505
    Purpose
    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a, U6
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175505-plasmid-map-sequence-id-345098

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  47. pP2A_NLS_sfGFP

    Plasmid
    #63709
    Purpose
    Expressing P2A_NLS_sfGFP with homologous arm of Nanog.
    Depositor
    Stanley Qi
    Article
    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.
    Insert
    Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
    Use
    Just a donor dna with nanog homologous arm for kn…
    Tags
    sfGFP
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63709-plasmid-map-sequence-id-107390

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Showing: 3001 - 3050 of 4441 results