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Showing: 3001 - 3050 of 18233 results

  14. LV TRE-GFP-TMD-DLL1

    Plasmid
    #194936
    Purpose
    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194936-plasmid-map-sequence-id-386565

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  16. pTFE3-donor

    Plasmid
    #112377
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP
    Depositor
    Kevin White
    Insert
    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112377-plasmid-map-sequence-id-229742

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  18. pMLX-donor

    Plasmid
    #112370
    Purpose
    CRISPR donor plasmid to tag human transcription factor MLX with GFP
    Depositor
    Kevin White
    Insert
    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112370-plasmid-map-sequence-id-229723

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  20. LEGO-pDHS-AP1x6-GM55

    Plasmid
    #217416
    Purpose
    Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter, and a chromatin priming element. Alias: LEGO-GM55-AP-1x6-pDHS(IL-3)
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217416-plasmid-map-sequence-id-434961

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  21. LEGO-pDHS-ITDE-AP1x6-DUSP5

    Plasmid
    #217419
    Purpose
    Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human DUSP5 promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217419-plasmid-map-sequence-id-434920

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  24. pSin4 UAP1 F383G

    Plasmid
    #169891
    Purpose
    Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation
    Depositor
    Jennifer Kohler
    Article
    Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10
    Insert
    UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine (F) at position 383 to glyc…
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169891-plasmid-map-sequence-id-333152

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  25. pTHRB-donor

    Plasmid
    #112333
    Purpose
    CRISPR donor plasmid to tag human transcription factor THRB with GFP
    Depositor
    Kevin White
    Insert
    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112333-plasmid-map-sequence-id-228563

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  26. pZNF445-donor

    Plasmid
    #112332
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP
    Depositor
    Kevin White
    Insert
    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112332-plasmid-map-sequence-id-228561

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  27. pZNF785-donor

    Plasmid
    #112317
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP
    Depositor
    Kevin White
    Insert
    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112317-plasmid-map-sequence-id-228521

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  32. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Dustin Maly
    Article
    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    226162-plasmid-map-sequence-id-450826

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  33. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  34. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  39. p1D4-hrGFP II

    Plasmid
    #71317
    Purpose
    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
    Depositor
    Belinda Chang
    Article
    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tags
    1D4 epitope (TETSQVAPA) and IRES-hrGFPII
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. mscv2.2-NAIP5

    Plasmid
    #60205
    Purpose
    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5
    Depositor
    Russell Vance
    Article
    Tenthorey et al Mol Cell. 2014 Apr 10;54(1):17-29. doi:
    Insert
    NAIP5
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60205-plasmid-map-sequence-id-96383

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Showing: 3001 - 3050 of 18233 results