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Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 CLV3
Plasmid#115485PurposeCRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK429
Plasmid#155376PurposePphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR305
Plasmid#124712PurposeExpression of uhpB under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
IG101: pMVP (L5-L4) TRE promoter
Plasmid#121729PurposepMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTRE promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101111PurposeEncodes dCas9(C)-VP64 with SAM components fused to AVPR2DepositorInsertAVPR2-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101106PurposeEncodes FKBP-dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-FKBP-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_2pabPAC
Plasmid#158969Purpose2P-activatable bPAC for AAVDepositorInsert2pabPAC
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-BTK-PH
Plasmid#67673PurposeGateway entry clone containing BTK-PH with no stop codonDepositorInsertBTK-PH-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
S3_004_026_MV1
Plasmid#134416PurposepYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
FdeR-N_2xNLS
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p3E-rab11al
Plasmid#109663PurposeMultisite gateway vector for 3' tagging with rab11alDepositorInsertrab11al (rab11al Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
907K
Plasmid#160236PurposeFlightN-PipR-VP16-Sv40-attBDepositorInsertspristinamycin repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI128
Plasmid#126209PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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p3E-rab6ba
Plasmid#109721PurposeMultisite gateway vector for 3' tagging with rab6baDepositorInsertrab6ba (rab6ba Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab6bb
Plasmid#109722PurposeMultisite gateway vector for 3' tagging with rab6bbDepositorInsertrab6bb (rab6bb Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only