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Plasmid#137755PurposeTargets Keima to the endoplasmic reticulum. Used to monitor ER-phagyDepositorInsertKeima-cb5
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-q ONE-GO
Plasmid#204331PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-q ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_luc-60A
Plasmid#200395PurposeA plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ3H PTP-1B
Plasmid#8601DepositorInsertPTP-1B (PTPN1 Human)
UseTagsHAExpressionMammalianMutationK103RPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z17-PDGFR_EF1a_mCherry
Plasmid#186303PurposePlasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ17-PDGFR
mCherry
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb-IRES-eGFP-W
Plasmid#32519DepositorInsertRheb (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
eTC GFP beta-actin full length
Plasmid#27123DepositorInsertbeta-actin (ACTB Human)
UseTagsEGFP and TC tagExpressionMammalianMutationE237APromoterAvailable sinceJan. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15
Plasmid#176212PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-cortactin
Plasmid#26722DepositorInsertCortactin (Cttn Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLC-HA-Nedd8-P2A-Hygro
Plasmid#124306PurposeLentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoterDepositorInsertNedd8 (NEDD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P28
Plasmid#120039PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBigTB-ERT2CreERT2
Plasmid#149433Purposetamoxifen-inducible Cre recombinaseDepositorInsertERT2-Cre-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
Plasmid#58442PurposeCassette to replace yeast promoters with an inducible promoter containing 4 lexA boxesDepositorInsertPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
UseTagsExpressionYeastMutationPromoterPTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Gal4DBD-GBP2
Plasmid#49439PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP2-Gal4 DNA binding domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorInsertAP180 (Snap91 Rat)
UseTagsFKBP N-terminal GFP C-terminalExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only