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Plasmid#98273Purposemammalian expression of TNFR1-tdEOSDepositorInsertTNFR1 (TNFRSF1A Human)
UseTagsFlag and tdEosExpressionMammalianMutationPromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-mutKir2.1
Plasmid#125579PurposeCre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationA non-conducting mutant (GYG -> AAA).PromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mIFP-H2B-6
Plasmid#56223PurposeLocalization: Nucleus/Histones, Excitation: 683, Emission: 704DepositorInsertH2B (H2BC11 Human)
UseTagsmIFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-BAP1-S63C-GFP
Plasmid#78911PurposeMammalian expression of BAP1 with S63E and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationS63CPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO- flag-hDDI2-D252N_pET11a
Plasmid#169123Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationD252NPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-flag-hDDI2_pET11a
Plasmid#169121Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
SHIP2 WT pBABE-His-Xpress
Plasmid#126584PurposeRetroviral infection of MEF cells (puromycin selection)DepositorInsertSHIP2 (INPPL1 Human)
UseRetroviralTagsHis Xpress (N-terminal)ExpressionMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-M11-92
Plasmid#134466PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mIFP-VASP-5
Plasmid#56241PurposeLocalization: Focal Adhesions, Excitation: 683, Emission: 704DepositorInsertVASP (Vasp Mouse)
UseTagsmIFPExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV106-pTDH3-mCherry-10xSumo-6xSIM
Plasmid#116928PurposeYeast integrative vector for expression of the SUMO-SIM complexDepositorInsert10xSUMO-6xSIM
UseTagsmCherryExpressionYeastMutationPromoterTDH3Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/RFP-DDX54
Plasmid#97061PurposeExpresses RFP (tdTomato)-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsRFP (tdTomato)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
LUC45 (L/Sec/mutP)
Plasmid#112148PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U) and SE…PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.RGS4.C-HA
Plasmid#154847PurposeExpresses murine RGS4 protein with HA-tag at C-terminusDepositorInsertRegulator of G protein Signaling 4 (Rgs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-L647RLaminA
Plasmid#69060Purposeencodes a lamin A mutant with Leucine 647 mutated to an ArgininDepositorInsertLaminA (LMNA Human)
UseRetroviralTagsExpressionMammalianMutationLamin A with Leucine 647 mutated to ArgininePromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH4R-P2A-mCherry-N1
Plasmid#84333PurposeCo-expresses (untagged) Histamine-4 receptor and mCherryDepositorInsertH4R-P2A-mCherry (HRH4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dzCas9-NG-Act3.0
Plasmid#158415PurposeCRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F
Plasmid#159132PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11FPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pyr pUAST-HA
Plasmid#69768PurposePyramus (FGF8-like-2) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPyramus (FGF8like-2) (pyr Fly)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutation241T amino acid insertion compared to reference s…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only