-
Plasmid#61460PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)DepositorInsertPK-hLC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-U6-3-t-attP40
Plasmid#133561PurposegRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructsDepositorInsertattP40 region guide RNAs
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12C
Plasmid#81667PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMito-LSSmKate1-N1
Plasmid#31878DepositorInserthuman cytochrome C oxidase subunit VIII (COX8A Human)
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-GFP
Plasmid#117242PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-FOS
Plasmid#33353DepositorInsertFOS (Fos Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative of c-Jun; heterodimerizes and i…PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT462
Plasmid#139393PurposeXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmRDepositorInsertXOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs
UseSynthetic BiologyTagsExpressionMutationPromoteraTC and BA inducible promotersAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 XBB Spike
Plasmid#195287PurposeExpresses codon-optimized full length SARS-CoV-2 XBB SpikeDepositorInsertSARS-CoV-2 XBB Spike (S )
UseTagsExpressionMammalianMutationContains the following mutations: T19I, LPPA24-27…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceFeb. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ratiometric FPX sensor for ERK kinase activity
Plasmid#60974PurposeFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.DepositorInsertddRFP A-WW domain-ERK substrate-ddFP B
UseTagsERK substrate is before ddFP copy B, WW domain is…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*-6HA
Plasmid#99520PurposeC-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags6xHAExpressionMutationPromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
eBFP2 (eBFP2)_CD
Plasmid#66034PurposeMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]DepositorInsertFluorescent reporter - BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SP
Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS-H2B-citrine
Plasmid#53752PurposeContains fusion of human Histone H2B to citrine.DepositorInserthistone H2B (H2BC21 Human)
UseTagscitrineExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
SFFV-SMASH-NTERM-GFP
Plasmid#185761PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, SMAShExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgHK2_2
Plasmid#163461Purposelentiviral vector expressing Cas9 and an sgRNA targeting HK2DepositorInsertsgRNA 2 targeting HK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorInsertHMGB1 (HMGB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG241 FKBP-CFAST10
Plasmid#130814PurposeExpresses FKBP-CFAST10 in mammalian cellsDepositorInsertFKBP-CFAST10
UseTagsMyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneUseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-EYFP
Plasmid#104052PurposeAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoterDepositorHas ServiceAAV PHP.V1InsertEYFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only