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Sar1b-mOrange2
Plasmid#166899Purposemammalian expression of Sar1b tagged with mOrange2DepositorInsertmOrange2
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
JAB2098
Plasmid#190340PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mApple-GPI-RUSH
Plasmid#166904PurposeRUSH cargo: Str-KDEL_IRES_SBP-mApple-GPIDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKEW106-MbCas12a-58069
Plasmid#115653PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Sox2-t2A-mCherry
Plasmid#127538PurposeExpresses human SOX2 and mCherry via t2A linker.DepositorInsertSOX2 (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pKEW336-MBP-TEV-MbCas12a-33362
Plasmid#115670PurposeExpresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purificationDepositorInsertMbCas12a
UseCRISPRTagsMBP-TEVExpressionBacterialMutationPromoterT7 RNAPAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
UseTags6X His and sortase A sorting motifExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBA1018
Plasmid#189587PurposeT4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing)DepositorInsertT4wtGT7 Deletion Locus 250bp Homology Arms
UseSynthetic BiologyTagsExpressionBacterialMutationEncodes for a full deletion of T4 from 52.1 (part…PromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_E299A
Plasmid#91855PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) E299A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationE299APromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A
Plasmid#91861PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) R0179A/K1080A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK1080APromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
SETMAR-Cas9
Plasmid#186701PurposePlasmid encoding SETMAR-Cas9 fusion under CMV promoterDepositorInsertSETMAR-Cas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K1087A
Plasmid#91864PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1087A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK1087APromoterAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas7/pHMGWA
Plasmid#81184PurposeExpresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
