Addgene

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pCI-neo Flag HAUSP

Plasmid

#16655

Depositor

Bert Vogelstein

Article

Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

Insert

HAUSP (USP7 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLuxB

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFLxR4

Plasmid

#167517

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Carbenicillin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLuxB

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGPI-SceI

Plasmid

#32060

Purpose

Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Type

Empty backbone

Use

Mutagenesis plasmid

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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6972 pPROEX-HTb-SSA1

Plasmid

#1231

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ssa1 (SSA1 Budding Yeast)

Use

Tags

his tag (tev cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pDmREDusk

Plasmid

#213701

Purpose

Vector for red-light-repressed bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDrDERusk

Plasmid

#213703

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAd5-B2

Plasmid

#122552

Purpose

Block 2 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 3737-10881

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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TJF229

Plasmid

#132575

Purpose

pZE1-oxySp-mCherry-proD-oxyR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
proD-oxyR-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

oxySp and proD

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTEC16

Plasmid

#30175

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mOrange2

Expression

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pFG7m34GW

Plasmid

#133747

Purpose

Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Use

Tags

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7

Plasmid

#64953

Purpose

mini-Tn7 base vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pKL2315

Plasmid

#187876

Purpose

Expresses Cre recombinase.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

J23107

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overespression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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ptac::ccdB

Plasmid

#15922

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

ccdB (ccdB E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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