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Plasmid#218590PurposeExpress peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationErg20(F96W, N127W), Erg20-Erg20 synthetic homodim…PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_MYC9
Plasmid#223897Purpose4G-cloning donor for C-terminal MYC9 tagDepositorInsertMyc9
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_FLAG3
Plasmid#223896Purpose4G-cloning donor for C-terminal FLAG3 tagDepositorInsertFLAG3
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_PK6
Plasmid#223898Purpose4G-cloning donor for C-terminal PK6 tagDepositorInsertPK6
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_FLAG
Plasmid#223895Purpose4G-cloning donor for C-terminal FLAG tagDepositorInsertFLAG
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_HALO
Plasmid#223899Purpose4G-cloning donor for C-terminal HALO tagDepositorInsertHALO
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6
Plasmid#223871Purpose4G-cloning donor for N-terminal His6 tagDepositorInsertHis6
UseGolden-gate donorTags6xHisExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_HALO
Plasmid#223885Purpose4G-cloning donor for N-terminal HALO tagDepositorInsertHALO
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP
Plasmid#223880Purpose4G-cloning donor for N-terminal GFP tagDepositorInsertGFP
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_His8
Plasmid#223886Purpose4G-cloning donor for C-terminal His8 tagDepositorInsertHis8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_TS
Plasmid#223891Purpose4G-cloning donor for C-terminal TwinStrep tagDepositorInsertTwinStrep
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
GGDestX2-Amp
Plasmid#157654PurposeGolden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNADepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterU6, CBhAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK1056
Plasmid#209546PurposeBTK Type 4 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 4 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTALYM4
Plasmid#47875PurposeDestination plasmid with mRuby2 for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTALYM9
Plasmid#47876PurposeDestination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsTy1tagExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_3'omega
Plasmid#223870Purpose4G-cloning donor for 3'-Gibson overhang, omega-sequenceDepositorInsert3'omega sequence and spacer
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-TEV
Plasmid#223872Purpose4G-cloning donor for N-terminal His6-TEV tagDepositorInsertHis6-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only