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Plasmid#215545PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-moxGFP-LMNB1
Plasmid#207772PurposeDonor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-mNeon-ACTB
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mNeon-ACTB
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_Tubb3 GFP(Y39TAG) KI
Plasmid#182678PurposeExpression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.DepositorUseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-FnCas12a-TLR-MCV1-gRNA
Plasmid#117412PurposeFnCas12a-gRNA targeting TLR2.0DepositorInsertFnCas12a gRNA targeting TLR 2.0
UseTagsExpressionMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-547-782
Plasmid#118376PurposeGateway compatible donor vector with KAT14-547-782 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 547-782)PromoterAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-KAT14-1-546
Plasmid#118377PurposeGateway compatible donor vector with KAT14-1-546 truncation.DepositorInsertKAT14 (KAT14 Human)
UseDonor-plasmidTagsExpressionMutationTruncation (aa 1-546)PromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)
Plasmid#191391PurposeDonor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(30-120)
UseCRISPR and Synthetic BiologyTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
Plasmid#191389PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(1-100)
UseTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>GAL80
Plasmid#104874PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.DepositorInsertT2A-GAL80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF219
Plasmid#72367PurposeDonor vector for 3' FLAG tag of human ZNF219DepositorInsertZNF219 Homology Arms (ZNF219 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TGIF2
Plasmid#72373PurposeDonor vector for 3' FLAG tag of human TGIF2DepositorInsertTGIF2 Homology Arms (TGIF2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF146
Plasmid#72359PurposeDonor vector for 3' FLAG tag of human ZNF146DepositorInsertZNF146 Homology Arms (ZNF146 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorInsertSSRP1 Homology Arms (SSRP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ntag
Plasmid#195401PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-K40R-short-UBASH3A-Ctag
Plasmid#195400PurposeHuman K40R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationK40RPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_PBX2
Plasmid#72356PurposeDonor vector for 3' FLAG tag of human PBX2DepositorInsertPBX2 (PBX2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only