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We narrowed to 13,188 results for: cas9

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GluN2A (px330:2-guideCas9)

Plasmid

#169428

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-pegRNA

Plasmid

#169857

Purpose

SauCas9 pegRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pX165-Cas9-HF1

Plasmid

#140565

Purpose

Expresses Cas9-HF1 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Use

Tags

Expression

Mutation

N497A R661A Q695A Q926A

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA pAS single_hspCas9

Plasmid

#193311

Purpose

Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA pAS dual_hspCas9

Plasmid

#193312

Purpose

Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Tags

Expression

Mutation

L1 intron insertion

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Sa-SauriCas9

Plasmid

#135967

Purpose

Expresses Sa-SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA2

Plasmid

#89720

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA2 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pX165-Sniper-Cas9

Plasmid

#140560

Purpose

Expresses Sniper Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Use

Tags

Expression

Mutation

F539S M763I K890N

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-BRD4ΔN

Plasmid

#200968

Purpose

induction of phase-separated condensate mediated by BRD4dN-IDR

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

BRD4dN

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ150-Cas9(dpiRNA)

Plasmid

#107940

Purpose

eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

Cas9(dpiRNA)

Use

Tags

Expression

Worm

Mutation

Promoter

eft-3

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUF1-dCas9-Sir2a

Plasmid

#122510

Purpose

Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Insert

dCas9-Sir2a

Use

CRISPR

Tags

3*FLAG

Expression

Mutation

dCas9(D10A,H840A)

Promoter

PfHsp86

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-Gsk3b(new)

Plasmid

#122341

Purpose

Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Gsk3b

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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