-
Plasmid#134778PurposecGMP FRET biosensorDepositorInsertThPDE5VV
UseTagscpVenus-Venus and mTurquoise2ExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-ECFP-Actb
Plasmid#169787PurposeAn actin FRET sensor. Must be expressed with Venus-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsECFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-p65-AD
Plasmid#138395PurposeRMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)DepositorInsertT2A-p65-AD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Mito-CaNARi
Plasmid#64851PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNARi
UseTagsCerulean, N-terminal 30 amino acids from DAKAP1, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LT3REV
Plasmid#111174PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorTypeEmpty backboneUseLentiviralTagsDsRed and VenusExpressionMutationPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-CaNARi
Plasmid#64852PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNARi
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZA-YFP-R5
Plasmid#106257PurposeExpresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertYellow fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of YFP af…ExpressionBacterialMutationPromoterpL-TetOAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pFX-mCherry-Sec61β
Plasmid#174468PurposeExpress mCherry with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR S155A
Plasmid#72702PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationS155APromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL
Plasmid#208849PurposeExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Vc-C
Plasmid#183158PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/Vn-C
Plasmid#183154PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT3REVIR
Plasmid#111176PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertYFP-SMASh(AI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(TI)
Plasmid#111501PurposeExpresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].DepositorInsertYFP-SMASh(TI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-Vc
Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only