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Addgene
Showing: 1181 - 1200 of 1572 results
  1. PA-DAD-wt

    Plasmid
    #53095
    Purpose
    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
    Insert
    LOV(wt)-6aa-DAD
    Use
    Tags
    6x His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    L47F and C49S in Venus
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pKK-mAmber-TEV

    Plasmid
    #105775
    Purpose
    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mAmber-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pKK-TEV-mAmber

    Plasmid
    #105792
    Purpose
    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mAmber
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1181 - 1200 of 1572 results