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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VC

Plasmid

#110648

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

HA and VC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMH0006

Plasmid

#135448

Purpose

Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

Depositor

Martin Kampmann, Jonathan Weissman

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

dCas9-BFP-KRAB

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-mScarlet-I-CD4-bla

Plasmid

#179442

Purpose

Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

Plasmid

#69905

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Use

Tags

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IRF9-STAT1C

Plasmid

#37543

Depositor

Curt Horvath

Article

Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

Insert

IRF-9 STAT1 C (IRF9, STAT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GP1Fc Cl13

Plasmid

#138599

Purpose

Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

Depositor

Annette Oxenius

Article

Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

Insert

LCMV GP1-Fc

Use

Tags

Fc fusion protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-SNAP-CD4-bla

Plasmid

#179447

Purpose

Donor vector to knock in SNAPtag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMP108 Nanog-Venus-2a-mCherry

Plasmid

#159743

Purpose

Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

Depositor

Sergi Regot

Article

Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

Insert

Nanog homeobox (Nanog Mouse)

Use

Mouse Targeting

Tags

Venus-2a-mCherry

Expression

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-HIPK3 Exon

Plasmid

#69888

Purpose

Expresses the human HIPK3 exon 2 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Use

Tags

Expression

Insect

Mutation

Expresses aa1-194 of HIPK3

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PB-PARP1–eGFP

Plasmid

#211552

Purpose

A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Use

Piggybac

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPRoff-v2.1

Plasmid

#167981

Purpose

Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)

Use

Tags

2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Luciferase-hvTK-bla

Plasmid

#179450

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-PBZ-VN

Plasmid

#110646

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

DDK and VN

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBZ-mRuby2

Plasmid

#110650

Purpose

expresses a PAR-binding (PBZ) domain fused to mRuby2

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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