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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSuper-PICK1 shRNA

Plasmid

#67522

Purpose

Expresses shRNA against PICK1

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1, Pick1 Rat, Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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EmptyTranscriptionalReporter

Plasmid

#62453

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::NeoR-bGHpA

Plasmid

#62437

Purpose

MXS_chaining vector with CMV::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2-Cterminal6xHis

Plasmid

#60386

Purpose

Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Use

Tags

6xHIS

Expression

Yeast

Mutation

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pJB028

Plasmid

#65469

Purpose

pTrc plasmid expressing p14*-TEV

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

p14*-TEV (TEVgp1 )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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hME-pOX

Plasmid

#178181

Purpose

Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

Depositor

Ming-Feng Tsai

Article

Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

Insert

human MCU-EMRE fusion protein

Use

Tags

1D4

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-EF1a-Puro

Plasmid

#127170

Purpose

PCR template for EF1a-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7deltaE3

Plasmid

#175748

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 25043-35938

Use

Adenoviral and Synthetic Biology

Tags

Expression

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-CMV-Puro

Plasmid

#127169

Purpose

PCR template for CMV-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Use

Tags

mEos3.2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-ICP8 (pSLIK1)

Plasmid

#113856

Purpose

For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Expression

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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JAF99.4

Plasmid

#45560

Depositor

Gregory Pazour

Article

Follit et al J Cell Biol. 2010 Jan 11;188(1):21-8. do

Insert

Pkhd1 (Pkhd1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains only the cytoplasmic tail (last 193 amin…

Promoter

CMV

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only