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pRetroX-TetOne-Puro-p62 K7A/D69A

Plasmid

#204549

Purpose

Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K7A/D69A

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP11

Plasmid

#180344

Purpose

mammalian expression of human SEPT9_i1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP10

Plasmid

#180345

Purpose

mammalian expression of human SEPT9_i3 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP10

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP11

Plasmid

#180346

Purpose

mammalian expression of human SEPT9_i3 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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mTet1 flox donor

Plasmid

#117147

Purpose

Making Tet1 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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mTet3 flox donor

Plasmid

#117146

Purpose

Making Tet3 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet3 (Tet3 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-EGFP

Plasmid

#84041

Purpose

3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 FL

Plasmid

#89835

Purpose

Express GATA3 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_His6_GluA2

Plasmid

#170453

Purpose

Expresses histidine tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

Hexa-histidine

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR-TNF

Plasmid

#153069

Purpose

Human TNF 3'UTR region cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

3' UTR region of TNF gene (TNF Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

Plasmid

#153071

Purpose

Lentiviral vector expressing human ZC3H12D mutated (D95N)

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

Mutated ZC3H12D (D95N) (ZC3H12D Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed D95 to N, known to abrogate the RNase enz…

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-wtYAP

Plasmid

#84009

Purpose

expresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-BHLHE40-IRES-ZsGreen1

Plasmid

#153061

Purpose

Lentiviral vector expressing human BHLHE40

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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