-
Plasmid#112332PurposeCRISPR donor plasmid to tag human transcription factor ZNF445 with GFPDepositorInsertZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
UseCRISPRTagsExpressionMutationrs78585428, hg38:3:44446218:C>T, hg38:3:444466…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELK3-donor
Plasmid#112322PurposeCRISPR donor plasmid to tag human transcription factor ELK3 with GFPDepositorInsertELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
UseCRISPRTagsExpressionMutationhg38:12:96266960:A>T, rs760335647, rs6538704PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Tet
Plasmid#104524PurposeLI resistance gene moduleDepositorInsertTetracycline resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Cml
Plasmid#104525PurposeLI resistance gene moduleDepositorInsertChloramphenicol resistance gene
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS_gpyrG2
Plasmid#90277Purpose(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9DepositorInsertgRNA (pyrg2)
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP1071-2
Plasmid#99502Purpose3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEVDepositorInsert3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK324
Plasmid#71838PurposeCloned region contains Acetobacter aceti 1023 purE geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK340
Plasmid#71837PurposeCloned region contains Acetobacter aceti 1023 purE(H59N) geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK346
Plasmid#71836PurposeCloned region contains Acetobacter aceti 1023 purE(H59D) geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL439
Plasmid#70258PurposeSource of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genesDepositorInsertpsbA promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD06 K. lactis telomerase RNA hairpin
Plasmid#61895PurposePlasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)DepositorInsertTelomerase RNA hairpin (nt 229-273)
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
LI D-E 11GFP
Plasmid#54430DepositorInsertFLAG tag + and short linker + superfolder GFP 11 domain
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D mOrange noATG
Plasmid#54441DepositorInsertmOrange without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NES KOZ a c1
Plasmid#54444DepositorInsertCPK17-N-terminal + NES for enhanced export
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D T-Saphire noATG
Plasmid#54438DepositorInsertT-saphhire without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D GFP noATG
Plasmid#54436DepositorInsertsGFP without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D Cerulean noATG
Plasmid#54439DepositorInsertCFP Cerulean without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D mCherry noATG
Plasmid#54440DepositorInsertmCherry without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LI C-D YFP noATG
Plasmid#54437DepositorInsertYFP Venus without Start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGF004
Plasmid#48845PurposeProvides a D-alanine resistance cassette as GreenGate module.DepositorInsertp35S::D-AlanineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition site in 35S promoter re…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGA002
Plasmid#48813PurposeProvides the AP3 promoter as GreenGate module.DepositorInsertAP3 promoter
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition site removed by substit…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CJ47 (U1 snRNA (U1;21d) 3G mutant)
Plasmid#17776DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
UseTagsExpressionMutation3G mutantPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
IVT-R2Tg_opt
Plasmid#223247PurposeIn vitro transcription of the optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-36: AAVS1-mEGFP (PGK)
Plasmid#114404PurposeHomology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH75
Plasmid#128224Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b crRNA backbone
Plasmid#103854PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas ServiceCloning Grade DNAInsertU6-PspCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAW62.YY1.FKBP.knock-in.mCherry
Plasmid#104370PurposeFor knocking in FKBP F36V P2A mCherry onto YY1DepositorInsertFKBP12(F36V) -P2A-mCherry
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-42:AAVS1-mTagRFPT-CAAX
Plasmid#107580PurposeHomology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cellsDepositorInsertAAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 9, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorInsertFirefly luciferase
UseLuciferase; Mrna ivtTagsExpressionMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN7HELIX
Plasmid#190662PurposeExpresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
UseTagsExpressionMammalianMutationnAniI = K227M, F80K, L232KPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-19:LAMP1-mEGFP
Plasmid#101782PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1DepositorInsertLAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TCARZ-CMV-GFP-PAmin-GF3-R4A22
Plasmid#203768PurposeFor production of template RNA for use in PRINT, GFP under CMV promoterDepositorInsertPRINT template RNA for GFP
UseIvt templateTagsExpressionMutationPromoterCMVAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168DepositorInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRTagsExpressionMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only