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pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G
Plasmid#167937PurposeInducible CRISPRa expressionDepositorInsertdxCas9(3.7)-VPR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-P4M-SidM
Plasmid#51471PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEcCas-2.0
Plasmid#190697PurposeConstitutive expressing of Cas9 and inducible expression of lambda RedDepositorInsertCas9 and Lamda Red system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdMCP-YFP
Plasmid#99151PurposeExpresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vectorDepositorInsertDimeric MS2-coat binding protein
UseLentiviralTagseYFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SUMO-PNGaseF
Plasmid#228498Purpose10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)DepositorInsertngl
UseTags10xHis-SUMO(Smt3)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_blasti
Plasmid#194067Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2
Plasmid#190181PurposeFluorescent reporter vector to visualize all of cell cycle phases.DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLS-mP-Luc
Plasmid#106253PurposeLuciferase lentivirus based enhancer assay vectorDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterDerived from pGL4.23Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
UseTagsGST and his6ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2A
Plasmid#17924DepositorInsertNMDAR2A (Grin2a Rat)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Tax WT
Plasmid#23284DepositorInsertTax
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only
