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Plasmid#128402PurposePeriplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertTetanus toxin light chain domain
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating E234A mutationPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HH-gRNA-HDV-Shuttle-Vector
Plasmid#169098PurposeBackbone plasmid to generate HH-gRNA-HDV segmentDepositorInsertHH-gRNAbackbone-HDV
UseTagsExpressionMammalianMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attR3
Plasmid#213054PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-GFP U6promoter
Plasmid#128049PurposeAdenoviral shuttle vector for shRNA expression from U6 promoter with GFP markerDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-IRES-GFP
Plasmid#128036PurposeAdenoviral shuttle vector with CMV promoter, coexpresses EGFPDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3
Plasmid#160252PurposeFor stable integration of FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle CMV RGECO-TnI
Plasmid#124642PurposeMyofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombinationDepositorInsertRGECO-TnI (TNNI3 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-GFP H1promoter
Plasmid#128050PurposeAdenoviral shuttle vector for shRNA expression from H1 promoter with GFP markerDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brm BDmut-Flag pShuttle
Plasmid#122108PurposeExpression of full length Brm containing point mutations in its bromodomain in mammalian cellsDepositorInsertBrm (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-METTL14-R298P
Plasmid#141119PurposeRetroviral overexpression of mutant METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationSubstitution - Missense, position 298, R➞PPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShuttle AMPK gamma1 H150R
Plasmid#15999DepositorInsertAMPK gamma1 (Prkag1 Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationH150RPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-CtoS-FLAG
Plasmid#220876PurposeExpressing METTL17-FLAG_Cys to Ser mutantsDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged Cys to SerPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-LYRMut-FLAG
Plasmid#220877PurposeExpressing METTL17-LYRMut-FLAGDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged the LYP domain to AAAPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3 APPA
Plasmid#207124PurposeLentiviral plasmid to express mouse Mettl3 APPA mutantDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationD395A and W398APromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)
Plasmid#160582Purpose35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)DepositorInsertattR:P35S:TMtb:attL
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Puro-TTL-TTF-1-HDD
Plasmid#74989PurposeHuman TTF-1 (NKX2-1) cDNA with the homeodomain deletedDepositorInsertTTF-1 HDD mutant (NKX2-1 Human)
UseRetroviralTagsExpressionMammalianMutationHomeodomainPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. GtoC mu…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV-F2A-T2A-Venus
Plasmid#62621PurposeAdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tagDepositorInsertMCS with F2A and T2A
UseAdenoviralTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-Mettl3-dsRed-IRES-puro
Plasmid#61516PurposeConstitutive expression of murine wild-type Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGGAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only