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Plasmid#27238DepositorInsertI-kappa-B kinase epsilon (IKBKE Human)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2 F184A
Plasmid#85659Purposeretroviral expression of human SMYD2 F184ADepositorInsertSMYD2 (SMYD2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationF184APromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 IKKE K38A flag
Plasmid#26202DepositorInsertI-kappa-B kinase epsilon K38A (IKBKE Human)
UseTagsFlagExpressionMammalianMutationCatalytic Lysine changed to Alanine (K38A)PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2
Plasmid#85658Purposeretroviral expression of human SMYD2DepositorInsertSMYD2 (SMYD2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MYRIP
Plasmid#89586PurposeExpression plasmid for a positive control prey protein in NanoSPD 2.0 assays.DepositorInsertMYRIP (Myrip Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTT3-SP-6xHis-NTRK1(FL)-FLAG
Plasmid#157620PurposeMammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assaysDepositorInsertNTRK1 (NTRK1 Human)
UseTags6xHis and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
UseTagsExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis
Plasmid#156581PurposeMammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertmCherry
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PILRA-COMP5AP-AviTag-9xHis
Plasmid#157142PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPILRA (PILRA Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF14-COMP5AP-AviTag-9xHis
Plasmid#157149PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFSF14 (TNFSF14 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO10-HMM
Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
UseTagsExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO7A-TAIL
Plasmid#89585PurposeExpression plasmid for a positive control bait protein in NanoSPD 2.0 assays.DepositorInsertMYO7A (Myo7a Mouse)
UseTagsEGFPExpressionMammalianMutationTruncation encoding amino acids 870-2166 of MYO7A…PromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
UseTagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis
Plasmid#157534PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3E (SEMA3E Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis
Plasmid#157541PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3F (SEMA3F Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3C-COMP5AP-AviTag-9xHis
Plasmid#157525PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorInsertSEMA3C (SEMA3C Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA6D-COMP5AP-AviTag-9xHis
Plasmid#157498PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSEMA6D (SEMA6D Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA4A-COMP5AP-AviTag-9xHis
Plasmid#157502PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSEMA4A (SEMA4A Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis
Plasmid#157052PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF12A (TNFRSF12A Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis
Plasmid#157187PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFSF12 (TNFSF12 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only