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Plasmid#198046PurposeMoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL1P3 ZmUbiP:GRF-GIF:NosT
Plasmid#198047PurposeMoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P4 ZmUbiP:GRF-GIF:NosT
Plasmid#198048PurposeMoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSGV Rh Acceptor
Plasmid#64271PurposeRetroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genesDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG014
Plasmid#113988PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 4DepositorInsertEndlinker/terminator Part 4
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG015
Plasmid#113989PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 5DepositorInsertEndlinker/terminator Part 5
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG013
Plasmid#113987PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 3DepositorInsertEndlinker/terminator Part 3
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG016
Plasmid#113990PurposeVector construction module. Endlinker/terminator module to circularize plasmid following Part 6DepositorInsertEndlinker/terminator Part 6
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG202
Plasmid#115503PurposeClone of a level 1 unit which encodes IPTG-inducible sfGFPDepositorInsertpL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG203
Plasmid#115504PurposeClone of a level 1 unit which encodes a constitutively-expressed mCherryDepositorInsertpL1M-F2-pNeo pOGG001, mCherry EC15071, T-pharma(pOGG003
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL1P6 ZmUbiP:GRF-GIF:NosT
Plasmid#198049PurposeMoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGExpressionMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I80_Bxb1mut(noBsaI)_GG1-GG4
Plasmid#219715PurposeLevel 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.DepositorInsertGG1-Bxb1(noBsaI0)-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSGV Hu Acceptor PGK-NGFR
Plasmid#64270PurposeRetroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFRDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0750
Plasmid#115511PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.DepositorInsertgusA pOGG083, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPS0377
Plasmid#115509PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFPDepositorInsertsfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0314
Plasmid#115507PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celBDepositorInsertcelB pOGG050, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterConstitutive promoter pNeo pOGG001Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0254
Plasmid#115506PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celBDepositorInsertRlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only