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pRSET FLIPPi-4u

Plasmid

#13553

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T163A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T163A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCas2F

Plasmid

#82377

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2C

Plasmid

#82374

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2D

Plasmid

#82375

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas61

Plasmid

#82397

Purpose

IPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

LDH

Use

Tags

Expression

Mutation

V39R

Promoter

cLac143

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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STREP-SUMO-FLAG-SeKaiC

Plasmid

#218179

Purpose

WT SeKaiC for phosphorylation/dephosphorylation study

Depositor

Andy LiWang

Article

Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

Insert

STREP-SUMO-FLAG-SeKaiC

Use

Tags

STREP-Tag

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.425

Plasmid

#203944

Purpose

Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a UP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.426

Plasmid

#203945

Purpose

Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a DOWN

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCas2B

Plasmid

#82373

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas7

Plasmid

#82379

Purpose

dCas9 expressed from low constituve promoter, c225, in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

c225

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2E

Plasmid

#82376

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas2A

Plasmid

#82372

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, A RBS: AGGAGA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas28

Plasmid

#82385

Purpose

sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23108

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas27

Plasmid

#82384

Purpose

sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PJ23117

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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RNA polymerase beta prime subunit FLAG

Plasmid

#102337

Purpose

Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

Depositor

Erin O'Shea

Article

Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

Insert

RNAP Beta Prime Subunit with C-terminal FLAG TAG

Use

Tags

3x FLAG with 3X GSGS linker

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#53300

Purpose

Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, crtB

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCas34

Plasmid

#82386

Purpose

sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Use

Tags

Expression

Mutation

Promoter

PEZ3

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMJ074

Plasmid

#215654

Purpose

pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

Depositor

Brian Pfleger

Article

Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

Insert

Yellow fluorescent protein

Use

Synthetic Biology

Tags

10x histidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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