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Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
InterTag
Plasmid#186910PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch
Plasmid#186906PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet
Plasmid#85578PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDBEcR/Bla
Plasmid#201556PurposePlasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.DepositorInsertDBEcR
UseUnspecified; Sleeping beauty transposonTagsMycExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-3xL
Plasmid#186909PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet
Plasmid#85577PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liverDepositorInsertCreER
UseCre/LoxTagsExpressionMammalianMutationPromoterApoE.HCR.hAATAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL
Plasmid#186913PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-2xL
Plasmid#186912PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)2 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)2-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-1xL
Plasmid#186911PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)1-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-2xL
Plasmid#186908PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)2 linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)2-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP in pDBEcR-pIND(SP1)/Bla
Plasmid#201557PurposeEcdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc and FLAG-EGFPExpressionMammalianMutationPromoterEF1a and pIMD(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC-ApoE-Tet-YAP
Plasmid#85580PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianMutationPromoterApoE.HCR.hAAT and TREAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet-YAP
Plasmid#85579PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianMutationPromoterTREAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2341_Tier3(SB)-Puro
Plasmid#169639PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2351_Tier3(SB)-Zeo
Plasmid#169648PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)DepositorInsertPRPBSA-driven ZeoR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2/LTR7-GFP
Plasmid#62541PurposeLTR7-driving reporterDepositorInsertLTR7-GFP
UseSleeping beauty transposonTagsExpressionMammalianMutationPromoterLTR7Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only