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Plasmid#125474PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh3-guide1
Plasmid#125473PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh2-guide4
Plasmid#125472PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh2-guide3
Plasmid#125471PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh2-guide1
Plasmid#125469PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh1-guide4
Plasmid#125468PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh1-guide2
Plasmid#125466PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 C2CD4A/B-enh1-guide1
Plasmid#125465PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mCherry-Tandem-PSAM4-GlyR
Plasmid#208361PurposeExpression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.DepositorInsertmCherry-TANDEM-PSAM4-GlyR
UseAAVTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPSeq-PP7-p65-HSF
Plasmid#196719PurposeCROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VP64
Plasmid#78901PurposeExpresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertdCas9-VP64
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro
Plasmid#180273PurposeLentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23b-proMMP-7
Plasmid#29524DepositorInsertproMMP-7 (MMP7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET23b-proMMP-7-E215Q
Plasmid#29642DepositorInsertproMMP-7 (MMP7 Human)
UseTagsExpressionBacterialMutationE215Q Catalytically InactivePromoterAvailable sinceApril 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180266PurposeLentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNADepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only