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Plasmid#184053PurposeLysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertLyso-InPAkt
UseTagsLysosome-associated membrane protein 1 (LAMP1).ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lyso-RA
Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-B
Plasmid#209873PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP B
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Lysosomes-20
Plasmid#57793PurposeLocalization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLAMP1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD50E in LAMP1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Lysosome-N-20
Plasmid#158006PurposeVisualization of lysosome in mammalian cells using mGoldDepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-PTEN(A4)-mCherry
Plasmid#184054PurposeLysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.DepositorInsertLyso-PTEN(A4)-mCherry (PTEN Human)
UseTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianMutationSer 380/385 and Thr 382/383 in PTEN mutated to Al…PromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
UseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lysosomes-20
Plasmid#55073PurposeLocalization: Lysosome Membrane, Excitation: 587, Emission: 610DepositorInsertLAMP1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Lysosomes-20
Plasmid#55246PurposeLocalization: Lysosome Membrane, Excitation: 383, Emission: 448DepositorInsertLAMP1
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
sfGFP-Lysosomes-20
Plasmid#56487PurposeLocalization: Lysosome Membrane, Excitation: 485, Emission: 507DepositorInsertLAMP1
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
YPet-Lysosomes-20
Plasmid#56636PurposeLocalization: Lysosome Membrane, Excitation: 513, Emission: 527DepositorInsertLAMP1
UseTagsYPetExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby-Lysosomes-20
Plasmid#55872PurposeLocalization: Lysosome Membrane, Excitation: 558, Emission: 605DepositorInsertLAMP1
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mApple-Lysosomes-20
Plasmid#54921PurposeLocalization: Lysosome Membrane, Excitation: 568, Emission: 592DepositorInsertLAMP1
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Emerald-Lysosomes-20
Plasmid#56476PurposeLocalization: Lysosome Membrane, Excitation: 487, Emission: 509DepositorInsertLAMP1
UseTagsEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTurboRFP-Lysosomes-20
Plasmid#58061PurposeLocalization: Lysosome Membrane, Excitation: 553, Emission: 576DepositorInsertLAMP1
UseTagstdTurboRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EYFP-Lysosomes-20
Plasmid#56595PurposeLocalization: Lysosome Membrane, Excitation: 513, Emission: 527DepositorInsertLAMP1
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-Lysosomes-20
Plasmid#58109PurposeLocalization: Lysosome Membrane, Excitation: 554, Emission: 581DepositorInsertLAMP1
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-Lysosomes-20
Plasmid#57284PurposeLocalization: Lysosome Membrane, Excitation: 503, Emission: 518DepositorInsertLAMP1
UseTagsDronpaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits