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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-GFP-TEV

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (u-msfGFP)

Plasmid

#29772

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

Plasmid

#86871

Purpose

To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

amino acids 7-29 deletion

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

Plasmid

#87203

Purpose

To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 108-130 are inserted.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Plasmid

#165030

Purpose

Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#160389

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection

Depositor

John McCusker

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Cen/ars

Tags

Expression

Yeast

Mutation

Promoter

Endogenous ATG8 promoter

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180359

Purpose

mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

Plasmid

#180361

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

Plasmid

#182231

Purpose

Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member B (RHOB Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Expression

Mutation

Promoter

TRE bidirectional

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

Plasmid

#182232

Purpose

Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member A (RHOA Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Expression

Mutation

Promoter

TRE bidirectional

Availability

Academic Institutions and Nonprofits only

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GFP-Rock1-GBD (840-110 aa) in pEGFPN1

Plasmid

#187279

Purpose

Express EGFP-Rock1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

GBD (84-110aa)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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human EB1 in pEGFP N1 to express EB1-GFP (JB131)

Plasmid

#39299

Depositor

Jennifer Tirnauer, Tim Mitchison

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

Plasmid

#74513

Purpose

AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

EGFP-p2A-EGFP-f

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET GFP domain 11 LIC cloning vector (GFP C-11)

Plasmid

#30183

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin-H6-TEV recognition and GFP domain 11

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

Plasmid

#180353

Purpose

mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

bidirectional TRE

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1

Plasmid

#180354

Purpose

mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN7 (SEPTIN7 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

bidirectional TRE

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

Plasmid

#180355

Purpose

mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN 9 (SEPTIN9 )
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Mutation

SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D

Promoter

bidirectional TRE

Availability

Academic Institutions and Nonprofits only

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