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Plasmid#223578PurposeProtein purification of human PD-L2 with SNAP and 10xHis tags in mammalian cellsDepositorInsertPD-L2 (PDCD1LG2 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-His6
Plasmid#223583PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CTLA-4-GCN4-His6
Plasmid#223582PurposeProtein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseTagsGCN4, His6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80 (I92R)-His10
Plasmid#223580PurposeProtein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD80 (CD80 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationI92RPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80-His10
Plasmid#223579PurposeProtein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD80 (CD80 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-CD3z
Plasmid#135993PurposeModular CD3z alone CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-BBz
Plasmid#135992PurposeModular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-Mart1-SABR
Plasmid#119052Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBIDepositorInsertA0201-Mart1-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G
Plasmid#194464PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-SABR-Backbone
Plasmid#119051Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-KK10-SABR
Plasmid#119053Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBIDepositorInsertB2705-KK10-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only