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Plasmid#228074PurposeMammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-EBFP2
Plasmid#228595PurposeMammalian expression of EBFP2, for MuSIC barcode constructionDepositorInsertEBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTagBFP2
Plasmid#228596PurposeMammalian expression of mTagBFP2, for MuSIC barcode constructionDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-EBFP2
Plasmid#228614PurposeMammalian expression of EBFP2 and extra insert, for MuSIC barcode constructionDepositorInsertEBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(S2)2(S4)2-gs
Plasmid#228072PurposeMammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(S2-S4)3-gs
Plasmid#228075PurposeMammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_SRR124.5_tagBFP
Plasmid#163753PurposegRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.DepositorInsertgRNA_SRR124.5
UseCRISPRTagstagBFPExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
gRNA_CloningVector_tagBFP
Plasmid#163747PurposeEmpty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.18_TagBFP
Plasmid#205533PurposeExpress TagBFPDepositorInsertTagBFP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKozak_mTagBFP2_synCoTC
Plasmid#199493PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
50_p5E_UBI_loxTagBFP
Plasmid#172518Purposep5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excisionDepositorInsertubiquitin promoter, TagBFP, loxP sites (inversion)
UseTagsExpressionMutationPromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_EBFP2
Plasmid#167889PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_EBFP2
Plasmid#167865PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorInsertpEBFP2-Nuc
UseTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFH3.2_mTagBFP2-MS2x12
Plasmid#205558PurposeExpress mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTagBFP2
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-mTagBFP2
Plasmid#184931PurposeTubulin labeling with blue fluorescent protein mTagBFP2DepositorInsertTUBA1B-mTagBFP2
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-Lifeact-7
Plasmid#54496PurposeLocalization: Actin, Excitation: 399, Emission: 456DepositorTypeEmpty backboneUseTagsmTagBFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only