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Addgene
Showing: 101 - 120 of 1520 results

  5. TFORF1914

    Plasmid
    #143553
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RUNX2 (RUNX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    143553-plasmid-map-sequence-id-400350

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  6. TFORF2914

    Plasmid
    #143669
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIB (NFIB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    143669-plasmid-map-sequence-id-400491

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  7. TFORF0914

    Plasmid
    #143450
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF836 (ZNF836 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    143450-plasmid-map-sequence-id-397892

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  9. pBP-SJM914

    Plasmid
    #72975
    Purpose
    Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    SJM914 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  11. pGBW-m4133914

    Plasmid
    #152552
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152552-plasmid-map-sequence-id-295564

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  12. pGBW-m4046914

    Plasmid
    #145738
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp9
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Availability
    Industry, Academic Institutions, and Nonprofits
    145738-plasmid-map-sequence-id-323301

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  13. pGBW-m4252914

    Plasmid
    #153588
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;TRX
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    153588-plasmid-map-sequence-id-300193

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  20. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

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Showing: 101 - 120 of 1520 results