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Showing: 251 - 300 of 358 results

  1. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Use
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  3. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
    Use
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

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  4. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

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  5. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  6. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  7. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  9. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

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  13. pCDF-B-pC

    Plasmid
    #213495
    Purpose
    Encodes Node B of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    Use
    Synthetic Biology
    Tags
    ASV tag and LVA tag
    Expression
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    213495-plasmid-map-sequence-id-430628

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  14. ρ1-EM GABAA receptor in pEZT-BM

    Plasmid
    #213072
    Purpose
    Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD
    Depositor
    Erik Lindahl
    Article
    Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.
    Insert
    Human GABAa rho1 receptor (GABRR1 Human)
    Use
    Bacmam, baculovirus, oocyte expression
    Tags
    Twin-Strep tag and superfolderGFP
    Expression
    Mammalian
    Mutation
    Encodes residues S58–L383 and D451–S479, separate…
    Promoter
    CMV and T7
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213072-plasmid-map-sequence-id-423917

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  15. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

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  16. pM1s3AsG

    Plasmid
    #137921
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    137921-plasmid-map-sequence-id-322876

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  18. pMM7

    Plasmid
    #201213
    Purpose
    Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.
    Depositor
    Alex Nielsen
    Article
    Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1
    Inserts
    sfGFP
    Spo0A right flank
    Spo0A left flank
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    F11V, T13P, N39D, A179A
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    201213-plasmid-map-sequence-id-405310

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  19. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pAM005

    Plasmid
    #170009
    Purpose
    Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PvirK
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    170009-plasmid-map-sequence-id-335956

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  22. pAM002

    Plasmid
    #170006
    Purpose
    Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PpmrD
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    170006-plasmid-map-sequence-id-335939

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  25. pVHed02

    Plasmid
    #82352
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid
    Depositor
    Richard Murray
    Article
    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    82352-plasmid-map-sequence-id-169038

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  34. pVHed07

    Plasmid
    #82350
    Purpose
    Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid
    Depositor
    Richard Murray
    Article
    Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do
    Inserts
    mKate2 RFP
    Superfolder GFP
    Temporal logic gate with TP901 and Bxb1 attachment sites
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    82350-plasmid-map-sequence-id-169046

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  36. pAM003

    Plasmid
    #170007
    Purpose
    Encodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PmgtC
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170007-plasmid-map-sequence-id-335948

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  37. pAM004

    Plasmid
    #170008
    Purpose
    Encodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PugtL
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170008-plasmid-map-sequence-id-335294

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  38. pAM006

    Plasmid
    #170010
    Purpose
    Encodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PpagC
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170010-plasmid-map-sequence-id-335938

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  39. pAM007

    Plasmid
    #170011
    Purpose
    Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1 and PslyB
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170011-plasmid-map-sequence-id-335739

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Showing: 251 - 300 of 358 results