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Plasmid#47683PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsdsRedExpressDRExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-NICD
Plasmid#26891DepositorInsertNICD (Notch1 Mouse)
UseTagsFLAG and b-globin polyAExpressionMammalianMutationE2299KPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLHTC Int3HA
Plasmid#21330DepositorInsertInt3 (Notch4 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBSSK Int3HA
Plasmid#21328DepositorInsertInt3 (Notch4 Mouse)
UseCloning vectorTagsHAExpressionMutationPromoterAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLNC Int3HA
Plasmid#21329DepositorInsertInt3 (Notch4 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceSept. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCLE NICD3
Plasmid#26894DepositorInsertNICD3 (NOTCH3 Human)
UseRetroviralTagsAlkaline Phosphatase and IRESExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-NICD
Plasmid#44471DepositorInsertNotch intracellular domain (Notch1 Mouse)
UseRetroviralTagsMycExpressionMammalianMutationContains amino acid 1753 to C-TermPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_ADAM17 site
Plasmid#188349PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a ADAM17site, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_ADAM17site_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-tdTomato_L3-L2
Plasmid#113708PurposeSingle pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.DepositorInsertCBF (notch dependent promoter)
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FAP site
Plasmid#188350PurposeLentiviral vector - constitutive expression of an myctag SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_myctag_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP
Plasmid#40640DepositorInsertNICD3 (NOTCH3 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD28 SNIPR
Plasmid#188358PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 2
Plasmid#188362PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)6_SNIPR
Plasmid#188344PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-empty-ires-EGFP
Plasmid#122953PurposeLentiviral expression empty vector under CAG promoter with EGFP expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_CBF-GLuc_L3-L2
Plasmid#113868PurposeSingle pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.DepositorInsertsCBF (notch dependent promoter)
GLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha SNIPR
Plasmid#188357PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 4
Plasmid#188364PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 2, G318A
Plasmid#188365PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_G318A, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_G318A_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)3_SNIPR
Plasmid#188343PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)3, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)3_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)9_SNIPR
Plasmid#188345PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)9, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)9_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)12_SNIPR
Plasmid#188346PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)12, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)12_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)15_SNIPR
Plasmid#188347PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)15, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)15_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)18_SNIPR
Plasmid#188348PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)18, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)18_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FAP site with scFv
Plasmid#188351PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_3xFLAG-tag tether
Plasmid#188352PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a 3X-FLAG-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_3X-FLAG-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_IgG4 SNIPR
Plasmid#188359PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a IgG4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_IgG4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_OX40 SNIPR
Plasmid#188360PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a OX40-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_OX40-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 3
Plasmid#188363PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant3-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant3-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 2, V319A
Plasmid#188366PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_V319A_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLX306 iCre tetO NICD rtTA
Plasmid#158049Purposelenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domainDepositorInsertmouse Notch Intra Cellular Domain
UseTagsV5ExpressionMammalianMutationPromotertetO promoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB-_attB53
Plasmid#183615PurposeRMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2321
Plasmid#169626PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2312
Plasmid#169622PurposeTier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NICD
Plasmid#61540PurposeExpresses NOTCH-ICD in mammalian cellsDepositorInsertNOTCH-ICD (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationNOTCH intracellular domain (aa1761-2555); A1943S…PromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVX
Plasmid#17666DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCX138
Plasmid#229213PurposeCRISPR array plasmid for NOTCH2 (Spacers 25-48)DepositorArticleInsertCRISPR array plasmid for NOTCH2 (Spacers 25-48)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCSegfpN5ICD
Plasmid#22467DepositorInsertNotch3 (notch3 Zebrafish)
UseInjection constructTagsExpressionMutationPromoterAvailable sinceMarch 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCX137
Plasmid#229212PurposeCRISPR array plasmid for NOTCH2 (Spacers 1-24)DepositorArticleInsertCRISPR array plasmid for NOTCH2 (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only