Skip to main content
Addgene
Showing: 151 - 180 of 6343 results

  1. LC501: pMVP (L3-L2) mCherry + polyA

    Plasmid
    #121765
    Purpose
    pMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    mCherry + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121765-plasmid-map-sequence-id-237249

    Enlarge

  4. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid
    #65529
    Purpose
    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
    Depositor
    Sebastian Bultmann
    Article
    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv
    Insert
    attB-mCherry
    Use
    Mouse Targeting; Bxb1
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65529-plasmid-map-sequence-id-111228

    Enlarge

  5. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

    Enlarge

  6. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

    Enlarge

  11. KZ701: pMVP (L3-L2) AID + polyA

    Plasmid
    #121791
    Purpose
    pMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to gene
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    AID + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121791-plasmid-map-sequence-id-237292

    Enlarge

  16. pAG426-GAL-3xFLAG-PolyS

    Plasmid
    #181728
    Purpose
    Galactose inducible expression of 3xFLAG-PolyS
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG-PolyS
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    181728-plasmid-map-sequence-id-468608

    Enlarge

  17. pAG426-GAL-3xFLAG-PolyA

    Plasmid
    #181726
    Purpose
    Galactose inducible expression of 3xFLAG-PolyA
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG-PolyA
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    181726-plasmid-map-sequence-id-468557

    Enlarge

  18. pAG426-GAL-3xFLAG-PolyL

    Plasmid
    #181727
    Purpose
    Galactose inducible expression of 3xFLAG-PolyL
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG-PolyL
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    181727-plasmid-map-sequence-id-468556

    Enlarge

  19. pAG426-GAL-3xFLAG-PolyC

    Plasmid
    #181729
    Purpose
    Galactose inducible expression of 3xFLAG-PolyC
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG-PolyC
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    181729-plasmid-map-sequence-id-468511

    Enlarge

  21. 3'dmDR-CMV-CasRx-bGHpolyA

    Plasmid
    #229556
    Purpose
    Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end
    Depositor
    Omar Akbari
    Insert
    CasRx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229556-plasmid-map-sequence-id-455012

    Enlarge

  22. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221452-plasmid-map-sequence-id-437434

    Enlarge

  23. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221453-plasmid-map-sequence-id-437437

    Enlarge

  24. 1203C-CMV-5’+3'DR-EGFP-bGHpolyA

    Plasmid
    #221454
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221454-plasmid-map-sequence-id-437443

    Enlarge

  25. 1203Q-5'dmDR-NT(22)-GFP-bGHpolyA

    Plasmid
    #221538
    Purpose
    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221538-plasmid-map-sequence-id-442639

    Enlarge

  26. 1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA

    Plasmid
    #221539
    Purpose
    EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221539-plasmid-map-sequence-id-437451

    Enlarge

  27. 1203M-CMV-dDiCas7-11-bGHpolyA

    Plasmid
    #220957
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220957-plasmid-map-sequence-id-437418

    Enlarge

  28. 1203P-CMV-Cas7.1-bGHpolyA

    Plasmid
    #220960
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector)
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220960-plasmid-map-sequence-id-437828

    Enlarge

Showing: 151 - 180 of 6343 results