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Addgene
Showing: 151 - 180 of 848 results
  1. pWPI-IRES-Bla-Ak-ACE2

    Plasmid
    #154981
    Purpose
    Lentiviral vector expressing Human ACE2
    Depositor
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pLENTI_hACE2_PURO

    Plasmid
    #155295
    Purpose
    Lentiviral vector to generate hACE2 stable expressing cell line
    Insert
    hACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    C9
    Expression
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pREG(R1-R4) #BGV059

    Plasmid
    #48991
    Purpose
    Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid
    #28289
    Purpose
    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  6. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo

    Plasmid
    #128452
    Purpose
    lentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

    Plasmid
    #128453
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene
    Depositor
    Insert
    Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo

    Plasmid
    #128454
    Purpose
    lentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

    Plasmid
    #128455
    Purpose
    lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo

    Plasmid
    #128463
    Purpose
    lentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pSMPUW_8.4_Ex_CAMKIIA-EGFP-V5-SV40-Zeo

    Plasmid
    #128464
    Purpose
    lentiviral expression vector with CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Insert
    CAMKIIA promoter, EGFP gene, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pUltra-Plus-H2B-EGFP

    Plasmid
    #161776
    Purpose
    3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest
    Insert
    H2B-EGFP from Addgene Plasmid #26790
    Use
    Lentiviral; Bicistronic
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    UbC and UbC promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCTā€¦
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  15. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  16. pLgw EcoDam-V5-ELK1Y158A

    Plasmid
    #98608
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Availability
    Academic Institutions and Nonprofits only
  17. pLgw ELK1Y158A-V5-EcoDam

    Plasmid
    #98609
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Availability
    Academic Institutions and Nonprofits only
  18. pTJK368

    Plasmid
    #138006
    Purpose
    BirA* expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Insert
    BirA*
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  19. pHR_SFFV*/Puro2a-PAG1-GFP

    Plasmid
    #198223
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Insert
    PAG1
    Use
    Lentiviral
    Tags
    Followed by EGFP, after a flexible linker and Preā€¦
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  20. pCDH-CB-iCre

    Plasmid
    #72257
    Purpose
    Express iCre under CB promoter
    Depositor
    Insert
    iCre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Availability
    Industry, Academic Institutions, and Nonprofits
  21. pTALEN(Nd154C63) SFFV

    Plasmid
    #50431
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Availability
    Academic Institutions and Nonprofits only
  22. pTALEN(Nd154C63) MND

    Plasmid
    #50432
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MND
    Availability
    Academic Institutions and Nonprofits only
  23. pTALEN(Nd154C63) Ef1a

    Plasmid
    #50433
    Purpose
    pTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  24. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid
    #82327
    Purpose
    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)
    Depositor
    Insert
    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  25. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  26. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  27. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 151 - 180 of 848 results