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Showing: 11961 - 11980 of 16226 results

  3. pJK413

    Plasmid
    #155372
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155372-plasmid-map-sequence-id-309000

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  4. pSB1A2QS

    Plasmid
    #138268
    Purpose
    MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity
    Depositor
    Xiao Wang
    Article
    Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Inserts
    AraC-TetR operon
    LuxR-LacI operon
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para/lac and Plux/tet
    Available since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    138268-plasmid-map-sequence-id-288132

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  6. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  7. pCoofy62

    Plasmid
    #122014
    Purpose
    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122014-plasmid-map-sequence-id-243584

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  11. BC-A1-014

    Plasmid
    #78702
    Purpose
    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78702-plasmid-map-sequence-id-171059

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  15. jk64

    Plasmid
    #87012
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    Nrx1B-4xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87012-plasmid-map-sequence-id-173035

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Showing: 11961 - 11980 of 16226 results