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Plasmid#107344PurposeExpresses UBE3B mutant C1036A fused to copGFP in mammalian cells.DepositorInsertUBE3B(C1036A)copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationmutation C1036APromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
Plasmid#45774DepositorInserttetR-deGFP
UseSynthetic BiologyTagsFusion protein of TetR and deGFP, with glycine-se…ExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-MCS-P2A-GFP-PuroR
Plasmid#107186PurposeBase plasmid for cloning gene duplication alleles with eGFP reporter.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP-SFPQY527A-IRES-mCherry
Plasmid#166951PurposeLentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsGFP and mCherryExpressionMutationSFPQ-Y527APromotereF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-eGFP NLS (no drug resistant gene)
Plasmid#140032PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-deGFP-8xHis-T500
Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP
Plasmid#182826PurposeCre-dependent expression of membrane localized APEXDepositorInsertLckAPEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP
Plasmid#200279PurposeGenetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivoDepositorArticleInsertzFn-FingR-GPHN-eGFP
UseAAVTagseGFPExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2
Plasmid#194515PurposeP2 promoter for transgenic enhancer assays in zebrafishDepositorInsertEnhanced Green Fluorescent Protein
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-G SCT-T2A-GFP
Plasmid#205462Purposelentiviral plasmid for expression of HLA-G SCTDepositorInsertHLA-G SCT (HLA-G Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse deltaPE Oct4-GFP transgenic reporter
Plasmid#52382PurposeBAC recombineering generated construct, used as a reporter for mouse Oct4 expressionDepositorInsertsUseTagsExpressionMammalianMutationdeleted -300 - -1300bp fragment upstream from Oct…PromoterOct4 and PGK+gb2Available sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAG-ABEmax-P2A-EGFP-ires-puro
Plasmid#121170PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.DepositorInsertABEmax-P2A-EGFP-ires-puro
UseCRISPRTagsP2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Membrin-IRES-Puromycin
Plasmid#134865PurposeExpresses EGFP-tagged Golgi apparatus markerDepositorInsertMembrin (GOSR2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SECURE BE3(R33A)-P2A-EGFP (pJUL1085)
Plasmid#123614PurposeCAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).DepositorInsertBE3(R33A)-P2A-EGFP
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in SpCas9PromoterCAGAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32476DepositorInsertPSAM Y115F,L141F:5HT3 HC
UseTagsIRES-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only