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pLV_TRE3G_hRTN3E-Halo

Plasmid

#216252

Purpose

Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon-3 isoform e (RTN3 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

TRE3G

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

Plasmid

#216253

Purpose

FRET-based calcium sensor located in the ER lumen, Tet-inducible

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

ER-targeted D4 second generation cameleon (FRET calcium sensor)

Use

Lentiviral

Tags

REAch and mTurquoise

Expression

Mutation

Promoter

TRE3G

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-APEX2-nDEST

Plasmid

#214992

Purpose

Gateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tag

Depositor

Wade Harper

Type

Empty backbone

Use

Lentiviral

Tags

Flag-APEX2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-cDEST-APEX2

Plasmid

#214993

Purpose

Gateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tag

Depositor

Wade Harper

Type

Empty backbone

Use

Lentiviral

Tags

APEX2-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-mCherry

Plasmid

#217005

Purpose

lentiviral plasmid for gRNA cloning with selectable mCherry expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-dsRed

Plasmid

#217006

Purpose

lentiviral plasmid for gRNA cloning with selectable dsRed expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S

Plasmid

#213591

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-S170D

Plasmid

#213592

Purpose

Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

S170D

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/D338N

Plasmid

#213594

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, D338N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/D338N

Plasmid

#213595

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, D338N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/Y280A

Plasmid

#213596

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, Y280A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/D321N

Plasmid

#213598

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, D321N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/Y263A

Plasmid

#213599

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, Y263A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 WT (PAM) _ Hygro

Plasmid

#213697

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

Depositor

Veerle Baekelandt

Article

Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

Insert

human ATP13A2 WT PAM (ATP13A2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT, D508N

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 P652_E653 del

Plasmid

#213700

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

Depositor

Veerle Baekelandt

Article

Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

Insert

human ATP13A2 P652_E653 del (ATP13A2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT, D962N

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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optoFcR

Plasmid

#224291

Purpose

Expression of an optogenetic Fc Receptor

Depositor

Meghan Morrissey

Article

Bond et al Dev Cell. 2024 Aug 9:S1534-5807(24)00457

Insert

Fc receptor gamma (Fcer1g Mouse)

Use

Lentiviral

Tags

mScarlet-Cry2-olig and myristoylation sequence

Expression

Mutation

aa 45-86 only

Promoter

SFFV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV(T9)-DERA-sgresis

Plasmid

#222622

Purpose

Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

DERA (DERA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-RNF8-R42A

Plasmid

#221702

Purpose

Lentiviral expression of human RNF8 (R42A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

R42A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-C403S

Plasmid

#221703

Purpose

Lentiviral expression of human RNF8 (C403S) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Tags

Expression

Mutation

C403S

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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