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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-RRAR

Plasmid

#164569

Purpose

SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-RRAR variant)

Use

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-Clathrin-15

Plasmid

#54040

Purpose

Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Clathrin (CLTB Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Cx43-msfGFP

Plasmid

#69007

Purpose

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Gja1 (Gja1 Rat)

Use

Tags

monomerized superfolder GFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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hRAD18 dSAP-EGFP

Plasmid

#68825

Purpose

Expresses human RAD18 (deleting SAP domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting SAP domain) tagged with EGFP (RAD18 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Use

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Atg14L

Plasmid

#21635

Depositor

Tamotsu Yoshimori

Article

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Insert

Atg14L (ATG14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWPXL-c-Myc

Plasmid

#36980

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

c-Myc (Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
PRPF19

Plasmid

#155847

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF19 (PRPF19 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.KAT5

Plasmid

#184647

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA AKT1

Plasmid

#78778

Purpose

To overexpress AKT1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Use

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3. Flag-CSL WT

Plasmid

#167004

Purpose

Mammalian expression of CSL WT

Depositor

Gian-Paolo Dotto

Article

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Insert

CSL (RBPJ Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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