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Showing: 11941 - 11960 of 16040 results

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rTetR-NS3-VP64-p65_IRES_mCherry

Plasmid

#112628

Purpose

Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

rTetR-NS3-VP64-p65

Use

Tags

AU1 (N term on NS3), HA (C term on NS3), and T7 (…

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Use

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Mutation

Promoter

Phyper spank

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal no NLS

Plasmid

#188397

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide

Expression

Bacterial

Mutation

Promoter

Phyper spank

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal NLS

Plasmid

#188398

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Mutation

Promoter

D-mannose

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Klf4Gata-3

Plasmid

#188401

Purpose

Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Klf4
Gata-3

Use

Tags

PhoD secretion peptide

Expression

Bacterial

Mutation

See Depositor Comments Below

Promoter

D-mannose

Availability

Academic Institutions and Nonprofits only

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pJE53

Plasmid

#194153

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

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pTS2311-Tier1-spacer-PPGK-NLuc_TB

Plasmid

#169552

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-NanoLuc Luciferase - mTagBFP fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Availability

Academic Institutions and Nonprofits only

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ULK1-Halo HRD

Plasmid

#207548

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Availability

Academic Institutions and Nonprofits only

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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Use

Tags

OsmY-10His-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'3'short

Plasmid

#129750

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Pgk

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2-Cb5

Plasmid

#18004

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

ER targeted Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminal of Bcl-2 replaced with Cytochrome b5. …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Flag-Bcl2-Maob

Plasmid

#18005

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C terminal of Bcl-2 replaced with Maob. Targets B…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only
mCardinal-Actin-C-18

Plasmid

#56151

Purpose

Localization: Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

NM_001101.3 (ACTB Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-Keratin-17

Plasmid

#56164

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Keratin (KRT18 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-LaminB1-10

Plasmid

#56165

Purpose

Localization: Nuclear Envelope, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits