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Plasmid#59143PurposeYAP1 mutants that harbor a deletion of the transcriptional activation domainDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTA (transactivation) domain deleted; aa291-497PromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-sCre
Plasmid#55635PurposeExpresses sCre in Mammalian CellsDepositorInsertCre
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-H169Q-GFP
Plasmid#78917PurposeMammalian expression of BAP1 with H169Q and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationH169QPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-C91G-GFP
Plasmid#78916PurposeMammalian expression of BAP1 with C91G and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationC91GPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD9
Plasmid#195847PurposeExpresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556HDepositorInsertFZD9 (FZD9 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS4-HA.SV40(polyA)
Plasmid#155193PurposeExpresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminusDepositorInsertThrombospondin-4 (Thbs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS1
Plasmid#170852PurposeAAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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nCas9-PPE
Plasmid#140445PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRTagsExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
UseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-mCherryTRF1-IRES-Blast
Plasmid#182588Purposeexpresses mcherry-trf1 in mammalian cellsDepositorInserthTRF1 (TERF1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _PDZ
Plasmid#59147PurposeYAP1 mutants that harbored a deletion of the 5 amino acidsDepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMammalianMutationPDZ binding domain (last 5aa) deletedPromoterCMVAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl
Plasmid#78712PurposeExpresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 2-85
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry GRK2 ct in pcDNA3.1
Plasmid#64212PurposemCherry fused between Cry2 and GRK2ct to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMV and cmvAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Ascl1
Plasmid#134671PurposeRetroviral expression of mouse Ascl1DepositorInsertAscl1 (Ascl1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-sr1(SIRPT1)
Plasmid#75297PurposeExpresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 84-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Ubl-sr1(SIRPT1)
Plasmid#75298PurposeExpresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 1-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only